Overcoming Challenges in Mammalian Shake Flask Cultures
Shake flask systems offer a flexible, scalable platform for mammalian cell research. However, scientists often face challenges that negatively affect culture performance, such as microbial contamination, uneven growth, and cell aggregation. Effectively addressing these factors is essential to achieving consistent and reliable experimental results.
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- Practical approaches to prevent microbial and eukaryotic contamination
- Optimizing shaking speed, fill volume, and environmental conditions
- Tools for monitoring culture health and ensuring reproducibility
